![]() Each test, which can be performed at room temperature, costs $4.67 to produce. Importantly, the technology is affordable and scalable. We envision this type of test being able to be used at high-populated locations such as schools, airports, stadiums, companies-or even in one's own home," said first author Marcelo Der Torossian Torres, Ph.D., a postdoctoral researcher at Penn. "Quick and reliable tests like RAPID allow for high-frequency testing, which can help identify asymptomatic individuals who, once they learn they are infected, will stay home and decrease spread. This is beneficial for detecting individuals at the earliest stages of infection, allowing for rapid care and the potential decrease of further viral spread. In addition, RAPID is able to detect COVID-19 at extremely low concentrations (1.16 PFU mL), which corresponds to a viral load that correlates with the initial stages of COVID-19 (about two to three days after onset of symptoms). For example, serological tests can take around 15 to 20 minutes, and they are about 60 to 70 percent accurate. RAPID provides results in four minutes, which is faster than most methods currently available for diagnosing COVID-19. For saliva samples, RAPID was 90 percent accurate. For the nasal swab samples, RAPID was 87.1 percent accurate. The team also analyzed 50 saliva samples from patients. In blinded tests, they analyzed 139 nasal swab samples-109 of which were COVID-19 positive and 30 COVID-19 negative, as determined by standard RT-PCR clinical assessments. The team assessed the performance of RAPID using both COVID-19 positive and negative clinical samples from the Hospital of the University of Pennsylvania, including samples of the highly contagious UK B117 variant. The signal can be read through a desktop instrument or a smartphone. That signal allows the test to discriminate between infected and healthy human samples. The RAPID technology uses electrochemical impedance spectroscopy (EIS), which transforms the binding event between the SARS-CoV-2 viral spike protein and its receptor in the human body, the protein ACE2 (which provides the entry point for the coronavirus to hook into and infect human cells), into an electrical signal that clinicians and technicians can detect. We believe inexpensive diagnostic tests like RAPID could help bridge some of those gaps." "We feel strongly about the health inequities witnessed during the pandemic, with testing access and the vaccine rollout, for example. We felt a responsibility to use our expertise to help-and the diagnostic space was ripe for improvements," de la Fuente said. "Prior to the pandemic, our lab was working on diagnostics for bacterial infections. The results can be read on a benchtop instrument or on a smartphone. An electrode printed using a screen printer-thousands of which can be printed in a day at very low cost-can detect the virus in nasal swab or saliva samples. RAPID was developed by a team led by César de la Fuente, Ph.D., a Presidential Assistant Professor in Psychiatry, Microbiology, Chemical and Biomolecular Engineering, and Bioengineering, to quickly and accurately detect the virus while remaining cheap enough to be widely accessible. These tests are also costly, they run a risk of cross-contamination, and can take hours or days to provide results. Though effective, the technique requires large laboratory space and trained workers to employ. Despite the urgency of the pandemic, most available methods for COVID-19 testing use RT-PCR-reverse transcription polymerase chain reaction-to detect SARS-CoV-2.
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